Witney lost 6-1 away to leaders Amersham & Chalfont in Middlesex, Berks, Bucks & Oxon Division 1 of the South League.
Rory Heaslip put Witney ahead with a fine individual strike, but they conceded two first-half goals when reduced to ten men by a green card for Ben Moss.
Just after half-time, the visitors went down to ten again when Heaslip was shown a yellow card. There was no way back after that as Witney let in four more goals.
On Sunday, skipper Andy Gosnell was sent off as Witney lost 3-1 to British Airways in the EHA Vase.
Fraser Bernard equalised to make it 1-1 at half-time, but BA netted twice in the second half.
Tom Hobbs, Will Briggs and Jonny Austin scored for Witney 2nd, who lost 5-3 at home to Bracknell 2nd in Division 4.
Witney 3rd won 3-2 at Wokingham thanks to two goals from Joe Taylor and one from Henry Grimmers.
Katie Oliver scored twice in the first half as Witney Ladies won 3-0 at home to Eastcote in South Clubs League Division 3A, with Helen Backhouse adding a third goal.
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